Year 13 Thinktank Visit
During our visit to Thinktank, Millennium Point on Thursday 26th April 2018, we carried out the polymerase chain reaction (PCR) and gel electrophoresis, which are topics from the last chapter in A-level Biology. The aim of the CSI investigation was to examine various DNA fragments and identify the ‘criminal’ from a number of suspects. However, only a small amount of DNA was left at the crime scene, which isn’t as useful in its minute quantity. Consequently, we carried out PCR to amplify the DNA thus producing an ample amount. For PCR to be successful, the DNA fragment, taq polymerase, primers and DNA nucleotides were all used. They were placed into the thermocycler, which sequentially varied its temperature in accordance with specific protocols to achieve amplification. Next, we carried out gel electrophoresis to match the various types of DNA with the one that was present at the ‘crime scene’. DNA was placed into wells in the gel and voltage applied to it which allowed the DNA fragments to separate according to their mass and charge. Using this process, we were able to correlate positions of DNA bands on the gel with the one from the crime scene. Taking part in this experiment was very useful as we gained a detailed insight into the practical aspects of chapter 21 using high quality equipment.
Maryam Shafiq 13AHA

Click here to view the photo gallery.











